This is one of the fun experiments since you’ll have perfectly formed beads and I can guarantee you’ll remember doing it. You might forget some of the details but that’s what we’re here for. 
Remember to write the experiment in your own words!
Equipment:
- Measuring Cylinder
- Sodium Alginate Solution
- Yeast Solution
- Calcium Chloride Solution
- Boiling Water Bath
- Fehlings I and II Solutions
- Two Test Tubes
- Stoppers
- Three Beakers
- Sieve
- Spoon
- Stirring Rod
- Retort Stand
- 2 x 1cm3 Syringe
- Large Syringe (with rubber tubing and clip)
Method:
- Place required amount of sodium alginate soultion into a beaker.
- Using a syringe, take a required amount of yeast solution and place in the same beaker as the sodium alginate solution.
- Stir the mixture with a stirring rod.
- Using another syringe, take a sample the mixture and add slowly, drop by drop into a beaker of calcium chloride.
- Allow to set and pour off liquid.
- Place beads into a sieve and rinse with cold water tap tp remove any traces of yeast cells.
- Check that the clip on the bottom of the large syringe is closed.
- Spoon beads into thte syringe on the retort stand after placing a beaker underneath to collect excess liquid.
- Pour equal volumes of collected solution and sucrose into separate test tubes and test both samples for reducing sugars, using Fehlings I and II and a boiling water bath.
Results
Before adding sucrose solution to immobilised enzyme => blue colour (no change)
After passing sucrose solution through immobilised enzyme => red/brown (reaction occurs)
